31 Jul In book: Shafer’s Textbook of Oral Pathology: 7th Edition, Edition: 7 th Edition, Chapter: Routine Histotechniques, Staining and Notes on. 5 Mar Histology refers to the study of microscopic structures in biological material and its ways where individual components are both structurally and. Histology Lab, Biology Spring Dr. Ed Devlin. Webpage for Course: Lab Topic.

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Foremost among these is Bouin’s solution. If there is no field diaphragm on your microscope set the substage condenser as high as it will go without actually touching the slide. There are common usages for fixatives in the bistotechniques laboratory based upon the nature of the fixatives, the type of tissue, and the histologic details to be demonstrated. When it dries out, it becomes explosive.

What would this structure look hiwtotechniques in another plane of section longitudinal, frontal, transverse, oblique?

Introductory Histotechniques

On some instruments there are red numbers. If you have problems mechanical or optical consult your instructor. Do not get in the habit of adjusting the intensity with the substage diaphragm vide infra.

Histotevhniques stomach primate sec. Methyl methacrylate is very hard and therefore good for embedding undecalcified bone. Mercurials and others are somewhere in between. The staining process makes use of a variety of dyes that have been chosen for their ability to stain various cellular components of tissue.

Tissues that come off the tissue processor are still in the cassettes and must be manually put into the blocks by a technician who must pick the tissues out of the cassette and pour molten paraffin over them.

Parotid gland human sec. Bone marrow red section. Gross examination consists of describing the specimen and placing all or parts of it into a small plastic cassette which holds the tissue while it is being processed to a paraffin block. Picrates include fixatives with picric acid.

We will use one of the common staining procedures, hematoxylin and eosin or the H and E staining procedure. There are three important necessities for proper sectioning: Feel free to ask for assistance or clarification during the tissue work-up.

Other dehydrants can be used, but have major disadvantages. Some of them have specialized applications, but are used very infrequently.


Areolar tissue spread film 6. Then, the next person using the cassette does not pay attention to the fact that there is tissue already in the cassette and puts his specimen in it. Thymus human infant sec. Once sections are cut, they are floated on a warm water bath that helps remove hisstotechniques. The sections are then ready for staining.

This should remove all traces of water. Try to determine what influence the diaphragm has on the details of the fibers appearance. Fixation and processing Sectioning Staining, both routine haematoxylin and eosin and special staining Correct use of the microscope Laboratory Safety Troubleshooting By the end of the course, participants should have gained practical skills in: On some Nikon microscopes early models there should be a circular piece of ground glass on top of the illuminator or attached to the underside of the substage condenser.

Histo Techniques

Allow to dry before examining. Another uses long chain aliphatic hydrocarbons Clearite. Sections are wrinkled or compressed.

Avoid mechanical damage to the microscope. At times during performance of surgical procedures, it is necessary to get a rapid diagnosis of a pathologic process. Examine unlabeled slides and micrographs or slides with the label covered. Buffering Penetration Volume Temperature Concentration Time interval Fixation is best carried out close to neutral pH, in the range of Sections are cut then removed from the blade with a small paint brush and placed on a slide covered with a thin layer of albumin and water 4 drops of water.

The microtome is nothing more than a knife with a mechanism for advancing a paraffin block standard distances across it.

Therefore, a variety of fixatives are available for use, depending on the type of tissue present and features to be demonstrated. Bone specimens are the most likely type here, but other tissues may contain calcified areas as well.

There are a variety of eosins that can be synthesized for use, varying in their hue, but they all work about the same. A product called paraplast contains added plasticizers that make the paraffin blocks easier for some technicians to cut.